calibrate_flu.Rd
Used by process_plate
function for fluorescence calibration. Function adds
calibrated fluorescence column to the data, which is returned. Originally
based on flopr::calibrate_flu
, but with multiple changes. A list of
arguments have been added to allow selection of required conversion factor
from table that may include conversion factors from multiple instruments,
FPs, etc, and function now includes error checks that report to the user if
conversion factors are missing.
calibrate_flu( pr_data, od_name, flu_instr, flu_channel, flu_gain, flu_slug, flu_label, do_quench_correction = do_quench_correction, conversion_factors_csv )
pr_data | a data.frame of parsed plate reader data |
---|---|
od_name | the column name for the optical density data |
flu_instr | instrument name |
flu_channel | fluorescent channel name |
flu_gain | gain |
flu_slug | name of fluorescent protein in FPbase slug format |
conversion_factors_csv | path of the csv file containing predicted conversion factors for the fluorescent channels |