Functions for performing calibrations

get_conc_A280()

Get FP concentrations using A280 method

get_conc_ECmax()

Get FP concentrations using ECmax method

get_conc_bca()

Get FP concentrations using BCA method

generate_cfs()

Generate conversion factors for FP calibrations

plot_absorbance_spectrum()

Plot absorbance spectrum

view_kfactors()

View k-factors

Experimental data handling functions

calc_fppercell()

Calculate FP/cell values

calc_fpconc()

Calculate FP concentration in molar units

process_plate()

Process timecourse plate reader data (normalise, correct and calibrate)

Parser functions

Functions that format and tidy data

magellan_parse()

Parser for Spark/Magellan data

magellan_spectrum_parse()

Parser for absorbance spectrum data

Helper functions

Functions that are used internally by other functions

calibrate_flu()

Convert arbitrary fluorescence units to calibrated units

calibrate_od()

Convert arbitrary optical density units to calibrated units

correct_flu()

Correct normalised fluorescence to compensate for cellular quenching effects

flu_norm()

Normalise fluorescence against negative well autofluorescence

get_extcoeff_A280()

Get a protein's molar extinction coefficient at A280

get_kfactor()

Get k-factor of a buffer

get_mw()

Get molecular weight of a protein

get_pathlength()

Get path length in a typical 96-well plate

get_properties()

Get properties of an FP from FPbase

od_norm()

Normalise optical density against blank wells

Data

aa_mass_data

Amino acid masses

cell_quench_data

cell quench data

fpbase_data

fluorescent protein properties from fpbase

kfactors_buffers_data

k-factors of different buffers

kfactors_temperature_data

k-factors of water at different temperatures

pathlength_water_data

pathlength vs volume data

protein_seq_data

protein sequence data