A dataset containing the k-factors (k-factor = A975-A900 at 1cm pathlength) of a range of aqueous buffers with salts and solvents, obtained from figure 5 of a Thermo Scientific application note on 'Microplate Based Pathlength Correction Method for Photometric DNA Quantification Assay' (Lampinen, Raitio, Perälä, Oranen and Harinen, 2012, ANMR_MUGO_0412). The dataset was extracted using WebPlotDigitizer (https://automeris.io/WebPlotDigitizer/), tidied to allow easier handling for programmatic purposes, and a column was added to calculate fold change in k-factors for all buffers compared to water.

kfactors_buffers_data

Format

A data frame with 10 rows and 6 variables:

buffer

name of the buffer

concentration

concentration of the buffer, units are variable

units

units of the concentration column, M is molar, pc is percentage ie. w/v (%)

description

string containing buffer-concentration-units information all in one

kfactor

kfactor value (A975-A900 at 1cm pathlength)

fold_change

fold change in kfactor from water

Source

https://static.thermoscientific.com/images/D20827~.pdf