Displays the data tables used for k-factor calculations in get_kfactors(), along with notes.
Examples
view_kfactors()
#>
#> Data from https://static.thermoscientific.com/images/D20827~.pdf :
#> buffer concentration units description kfactor fold_change
#> 1 water 0.00 none Water 0.173 1.0000000
#> 2 Na_phosphate 0.10 M Na_phosphate_100mM 0.170 0.9826590
#> 3 NaOH 0.02 M NaOH_20mM 0.169 0.9768786
#> 4 sulphuric_acid 0.02 M sulphuric_acid_20mM 0.169 0.9768786
#> 5 TE_buffer 0.01 M TE_buffer_10mM 0.169 0.9768786
#> 6 NaCl 0.90 pc NaCl_0.9% 0.168 0.9710983
#> 7 TBS 0.05 M TBS_50mM 0.166 0.9595376
#> 8 Na_phosphate 0.08 M Na_phosphate_80mM 0.166 0.9595376
#> 9 Tris-HCl 1.00 M Tris-HCl_1M 0.157 0.9075145
#> 10 Tween 0.10 pc Tween_0.1% 0.155 0.8959538
#> 11 sulphuric_acid 2.00 M sulphuric_acid_2M 0.154 0.8901734
#> 12 DMSO 1.00 pc DMSO_1% 0.169 0.9768786
#> 13 DMSO 5.00 pc DMSO_5% 0.159 0.9190751
#> 14 DMSO 10.00 pc DMSO_10% 0.148 0.8554913
#> 15 EtOH 5.00 pc EtOH_5% 0.159 0.9190751
#> 16 EtOH 20.00 pc EtOH_20% 0.129 0.7456647
#> 17 EtOH 40.00 pc EtOH_40% 0.092 0.5317919
#> 18 MeOH 5.00 pc MeOH_5% 0.161 0.9306358
#> 19 MeOH 20.00 pc MeOH_20% 0.133 0.7687861
#> 20 MeOH 40.00 pc MeOH_40% 0.100 0.5780347
#>
#> temperature kfactor fold_change
#> 1 25 0.172 1.000
#> 2 28 0.174 1.012
#> 3 31 0.177 1.029
#> 4 34 0.179 1.041
#> 5 37 0.183 1.064
#> 6 41 0.188 1.093
#> 7 45 0.191 1.110
#>
#> The k-factor of water (A975-A900 at 1cm) at ~25oC is 0.172.
#> Increasing the temperature has a small effect: 30oC = 3% increase to 0.176.
#> The volume of water is minimally effected by temperature, so path lengths calculated at 25oC should apply to other temperatures.
#>
#> Most aqueous buffers with low salt will be ~ 0.170 and can therefore be approximated to the k-factor of pure water.
#> eg.
#> 153mM (0.9%) NaCl = 0.168, TE = 0.169, 50mM TBS = 0.166
#> 1M Tris-HCl = 0.157, 2M H2SO4 = 0.154
#>
#> Other solvents, like DMSO, EtOH and MeOH have a bigger effect.
#> eg.
#> 10% DMSO = 0.148
#> 20% EtOH = 0.092, 20% MeOH = 0.100
#> 100% EtOH and MeOH have negative k-factors, and k-factors with these solvents are no longer reliable methods for calculating path lengths.