A dataset containing the k-factors (k-factor = A975-A900 at 1cm pathlength) of water at different temperatures, obtained from figure 6 of a Thermo Scientific application note on 'Microplate Based Pathlength Correction Method for Photometric DNA Quantification Assay' (Lampinen, Raitio, Perälä, Oranen and Harinen, 2012, ANMR_MUGO_0412). The dataset was extracted using WebPlotDigitizer (https://automeris.io/WebPlotDigitizer/), and a column was added to calculate fold change in k-factors for temperatures compared to 25oC.

kfactors_temperature_data

Format

A data frame with 10 rows and 6 variables:

temperature

temperature in oC

kfactor

kfactor value (A975-A900 at 1cm pathlength)

fold_change

fold change in kfactor from 25oC

Source

https://static.thermoscientific.com/images/D20827~.pdf