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Calculates the path length of an input test_volume by comparing it to a linear model using internal data of pathlengths vs volume. ... To work out the pathlength in a microplate assay, there are two options. Option 1: Measure the pathlength each time you do an assay by taking A975-A900 for each well as the kfactor(well), and calculating pathlength = kfactor(well) / kfactor(1cm pathlength). The 1cm k-factors can be derived using get_kfactor() function. Option 2 (used here): Create a standardised dataset of pathlength vs volume by measuring the A975-A900 of defined volumes of buffer, and calculating the pathlengths of each (the pathlength_water_data dataset) and use this to create a linear model of pathlength vs volume. Experimental data suggests that aqueous buffers give very similar pathlength values for a given volume, so here we use data from water to approximate all aqueous buffers.

Usage

get_pathlength(test_volume, plot = FALSE, outfolder = ".")

Arguments

test_volume

numeric value of volume whose pathlength is required, in microlitres (ul)

plot

logical. Should the function plot the model and prediction? Defaults to FALSE.

outfolder

path to folder where output files should be saved. Defaults to current working directory.

Examples

get_pathlength(200)
#> [1] 0.6072014