get_pathlength.RdCalculates the path length of an input test_volume by comparing it to a linear model using internal data of pathlengths vs volume. ... To work out the pathlength in a microplate assay, there are two options. Option1. Measure the pathlength each time you do an assay by taking A975-A900 for each well as the kfactor(well), and calculating pathlength = kfactor(well)/kfactor(1cm pathlength). The 1cm kfactors can be derived using get_kfactor function. Option2 (here). Use a standardised dataset of pathlength vs volume by measuring the A975-A900 of defined volumes of buffer, and calculating the pathlengths of each. This is saved as the pathlength_water_data dataset, and is used in this function to create a linear model of pathlength vs volume. Experimental data suggests that aqueous buffers give very similar pathlength values for a given volume, so here we use data from water to approximate all aqueous buffers.
get_pathlength(test_volume, plot = FALSE, outfolder = ".")
| test_volume | numeric value of volume whose pathlength is required, in microlitres (ul) |
|---|---|
| plot | logical. Should the function plot the model and prediction? Defaults to FALSE. |
| outfolder | path to folder where output files should be saved. Defaults to current working directory. |
get_pathlength(200)