correct_flu.RdUsed by process_plate function for cell quench correction. The presence of
cells, particularly at high densities, can 'quench' fluorescence such that
fluorescence readings are lower than expected from the equivalent FP
concentration in vitro. This function compensates for this effect using
empirical data that relates the fold decrease in fluorescence expected, given
a certain cell density.
correct_flu(pr_data, od_type, flu_channel)
| pr_data | a data.frame of parsed plate reader data |
|---|---|
| od_type | Which OD-type was used? Required for quench correction. "OD600" or "OD700". |
| flu_channel | fluorescent channel name |