Normalise fluorescence against negative well autofluorescence

Used by process_plate function for fluorescence normalisation. Remains virtually unchanged from flopr::flu_norm, except that af_model can be set to NULL to normalise to blank wells instead, and files are saved to a specified outfolder.

flu_norm(
  pr_data,
  neg_well,
  blank_well,
  flu_name,
  af_model,
  data_csv,
  outfolder
)

Arguments

pr_data	a long data.frame containing you plate reader data with OD normalised
neg_well	the well coordinates of a non-fluorescent control
blank_well	the well coordinates of a media blank
flu_name	the column name of the fluorescence chanel to normalise
af_model	model used to fit negative control autofluorescence. For now these include "polynomial", "invers_poly", "exponential", "spline" or "loess". If set to NULL, no model is used, and fluorescence is normalised akin to OD: by subtracting the value for the blanks.
data_csv	path to the original data. Used for saving normalisation curve plots.
outfolder	path to folder where output files should be saved. Defaults to current working directory.

Value