get_extcoeff_A280.Rd
The purpose of this function is to work out the theoretical molar extinction coefficient of a protein at 280nm (EC280, M-1cm-1) using only the protein's primary sequence, using the ProtParam method (Pace values). A full explanation of the method can be found at https://web.expasy.org/protparam/protparam-doc.html
get_extcoeff_A280( protein, disulphides = FALSE, showWarnings = TRUE, showMessages = TRUE, protein_name = "-", buffer = "-", mol_weight = NULL, save = TRUE, outfolder = "." )
protein | character string of protein sequence using 1-letter code |
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disulphides | logical. Does protein have disulphides? |
showWarnings | logical. Should function show warnings? |
showMessages | logical. Should function show messages? |
protein_name | character string of protein name. Optional. |
buffer | character string of buffer. Optional. |
mol_weight | numerical value for molecular weight (g/mol). Optional. If specified the function gives extinction coefficients for 1% (10mg/ml) and 0.1% (1mg/ml) solutions too. |
save | logical. Should function save csv file of output? |
outfolder | path to folder where output files should be saved. Defaults to current working directory. |